The selection of single-domain antibody fragments (iDAbs) by intracellular antibody capture in yeast
نویسندگان
چکیده
منابع مشابه
Selection of antibody fragments by yeast display.
The critical need for renewable, high-quality affinity reagents in biological research, as well as for diagnostic and therapeutic applications, has required the development of new platforms of discovery. Yeast display is one of the main methods of in vitro display technology with phage display. Yeast display has been chosen by numerous groups to refine both affinity and specificity of antibodie...
متن کاملOver expression of anti-MUC1 single-domain antibody fragments in the yeast Pichia pastoris.
The methylotrophic yeast Pichia pastoris has become a highly popular expression host system for the recombinant production of a wide variety of proteins, such as antibody fragments. Camelids produce functional antibodies devoid of light chains and constant heavy-chain domain (CH1). The antigen binding fragments of such heavy chain antibodies are therefore comprised in one single domain, the so-...
متن کاملBiotechnological applications of recombinant single-domain antibody fragments
BACKGROUND Single-domain antibody fragments possess structural features, such as a small dimension, an elevated stability, and the singularity of recognizing epitopes non-accessible for conventional antibodies that make them interesting for several research and biotechnological applications. RESULTS The discovery of the single-domain antibody's potentials has stimulated their use in an increa...
متن کاملSingle-domain antibody fragments with high conformational stability.
A variety of techniques, including high-pressure unfolding monitored by Fourier transform infrared spectroscopy, fluorescence, circular dichroism, and surface plasmon resonance spectroscopy, have been used to investigate the equilibrium folding properties of six single-domain antigen binders derived from camelid heavy-chain antibodies with specificities for lysozymes, beta-lactamases, and a dye...
متن کاملCloning and Expression of Recombinant Camelid Single-Domain Antibody in Tobacco
Antibodies provide a suitable tool in fundamental research and their high affinity and specificity make them invaluable for diagnostic and therapeutic applications. A promising alternative to conventional antibodies are the heavy chain antibodies (VHH) of Camelidae having short length, high solubility and stability are preferred to other antibody derivatives. In this study, our goal was product...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2018
ISSN: 2043-0116
DOI: 10.1038/protex.2018.007